[HTML][HTML] Influence of the cytosolic carboxyl termini of human B1 and B2 kinin receptors on receptor sequestration, ligand internalization, and signal transduction
A Faussner, D Proud, M Towns, JM Bathon - Journal of Biological …, 1998 - ASBMB
To determine the role of the cytoplasmic carboxyl termini of human B1 and B2 kinin
receptors (B1KR and B2KR, respectively) in the internalization of their respective ligands,
des-Arg 10-kallidin and bradykinin (BK), both wild type receptors, as well as truncated
B2KRs, a mutated B2KR, and chimeric receptors were stably expressed in Chinese hamster
ovary cells. Incubation of [3 H] BK at 37° C with cells expressing wild type B2KR resulted in
pronounced and rapid ligand internalization (∼ 80% after 10 min). By contrast, incubation of …
receptors (B1KR and B2KR, respectively) in the internalization of their respective ligands,
des-Arg 10-kallidin and bradykinin (BK), both wild type receptors, as well as truncated
B2KRs, a mutated B2KR, and chimeric receptors were stably expressed in Chinese hamster
ovary cells. Incubation of [3 H] BK at 37° C with cells expressing wild type B2KR resulted in
pronounced and rapid ligand internalization (∼ 80% after 10 min). By contrast, incubation of …