A rapid and cost-effective method for genotyping genome-edited animals: a heteroduplex mobility assay using microfluidic capillary electrophoresis

V Chenouard, L Brusselle, JM Heslan, S Remy… - Journal of Genetics and …, 2016 - Elsevier
The recent emergence and application of engineered endonucleases have led to the
development of genome editing tools capable of rapidly implementing various targeted
genome editions in a wide range of species. Moreover, these novel tools have become
easier to use and have resulted in a great increase of applications. Whilst gene knockout
(KO) or knockin (KI) animal models are relatively easy to achieve, there is a bottleneck in the
detection and analysis of these mutations. Although several methods exist to detect these …