[HTML][HTML] Sustained endothelial nitric-oxide synthase activation requires capacitative Ca2+ entry
S Lin, KA Fagan, KX Li, PW Shaul, DMF Cooper… - Journal of Biological …, 2000 - ASBMB
Endothelial nitric-oxide synthase (eNOS), a Ca 2+/calmodulin-dependent enzyme, is critical
for vascular homeostasis. While eNOS is membrane-associated through itsN-myristoylation,
the significance of membrane association in locating eNOS near sources of Ca 2+ entry is
uncertain. To assess the Ca 2+ source required for eNOS activation, chimera containing the
full-length eNOS cDNA and HA-tagged aequorin sequence (EHA), and MHA (myristoylation-
deficient EHA) were generated and transfected into COS-7 cells. The EHA chimera was …
for vascular homeostasis. While eNOS is membrane-associated through itsN-myristoylation,
the significance of membrane association in locating eNOS near sources of Ca 2+ entry is
uncertain. To assess the Ca 2+ source required for eNOS activation, chimera containing the
full-length eNOS cDNA and HA-tagged aequorin sequence (EHA), and MHA (myristoylation-
deficient EHA) were generated and transfected into COS-7 cells. The EHA chimera was …
