Peripheral blood B cells producing donor-specific HLA antibodies in vitro
M Han, JA Rogers, B Lavingia, P Stastny - Human immunology, 2009 - Elsevier
Donor-specific HLA antibodies have been associated with acute and chronic rejection. Such
antibodies may sometimes not be detected in recipient serum. In an attempt to learn about
possible mechanisms, we investigated antibody production by recipient B lymphocytes in
vitro. Peripheral blood B cells were obtained from 36 subjects, including 16 allograft
recipients, 12 sensitized patients, three multiparous women with serum HLA antibodies, and
five healthy non-transfused male subjects. Purified B cells were cultured with a cell line …
antibodies may sometimes not be detected in recipient serum. In an attempt to learn about
possible mechanisms, we investigated antibody production by recipient B lymphocytes in
vitro. Peripheral blood B cells were obtained from 36 subjects, including 16 allograft
recipients, 12 sensitized patients, three multiparous women with serum HLA antibodies, and
five healthy non-transfused male subjects. Purified B cells were cultured with a cell line …