Perfect seed pairing is not a generally reliable predictor for miRNA-target interactions
D Didiano, O Hobert - Nature structural & molecular biology, 2006 - nature.com
D Didiano, O Hobert
Nature structural & molecular biology, 2006•nature.comWe use Caenorhabditis elegans to test proposed general rules for microRNA (miRNA)-
target interactions. We show that G· U base pairing is tolerated in the'seed'region of the lsy-6
miRNA interaction with its in vivo target cog-1, and that 6-to 8-base-pair perfect seed pairing
is not a generally reliable predictor for an interaction of lsy-6 with a 3′ untranslated region
(UTR). Rather, lsy-6 can functionally interact with its target site only in specific 3′ UTR
contexts. Our findings illustrate the difficulty of establishing generalizable rules of miRNA …
target interactions. We show that G· U base pairing is tolerated in the'seed'region of the lsy-6
miRNA interaction with its in vivo target cog-1, and that 6-to 8-base-pair perfect seed pairing
is not a generally reliable predictor for an interaction of lsy-6 with a 3′ untranslated region
(UTR). Rather, lsy-6 can functionally interact with its target site only in specific 3′ UTR
contexts. Our findings illustrate the difficulty of establishing generalizable rules of miRNA …
Abstract
We use Caenorhabditis elegans to test proposed general rules for microRNA (miRNA)-target interactions. We show that G·U base pairing is tolerated in the 'seed' region of the lsy-6 miRNA interaction with its in vivo target cog-1, and that 6- to 8-base-pair perfect seed pairing is not a generally reliable predictor for an interaction of lsy-6 with a 3′ untranslated region (UTR). Rather, lsy-6 can functionally interact with its target site only in specific 3′ UTR contexts. Our findings illustrate the difficulty of establishing generalizable rules of miRNA-target interactions.
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